Journal: IET Nanobiotechnology

Article Title: The role of size in PEGylated liposomal doxorubicin biodistribution and anti‐tumour activity

doi: 10.1049/nbt2.12094

Figure Lengend Snippet: In vitro cytotoxicity effect (IC50) of different PEGylated nanoliposomal formulations, Caelyx® and free Dox against C26 Cells after 48h. Data represented as μ molar ± standard deviation ( n = 3)

Article Snippet: C26 mouse colon carcinoma cells were acquired from Cell Lines Service (Eppelheim, Germany), and they were cultured in Stands for Roswell Park Memorial Institute Medium (RPMI) 1640 medium (Sigma‐Aldrich).

Techniques: In Vitro, Standard Deviation

Journal: IET Nanobiotechnology

Article Title: The role of size in PEGylated liposomal doxorubicin biodistribution and anti‐tumour activity

doi: 10.1049/nbt2.12094

Figure Lengend Snippet: In vitro cellular uptake of different PEGylated nanoliposomal formulations, Caelyx® and free Dox by C26 cells at 37°C. Results expressed as geometric mean fluorescence intensity (MFI) ( n = 3, mean ± SEM)

Article Snippet: C26 mouse colon carcinoma cells were acquired from Cell Lines Service (Eppelheim, Germany), and they were cultured in Stands for Roswell Park Memorial Institute Medium (RPMI) 1640 medium (Sigma‐Aldrich).

Techniques: In Vitro, Fluorescence

Journal: IET Nanobiotechnology

Article Title: The role of size in PEGylated liposomal doxorubicin biodistribution and anti‐tumour activity

doi: 10.1049/nbt2.12094

Figure Lengend Snippet: Therapeutic efficacy of different PEGylated nanoliposomal formulations, Caelyx® and free Dox in BALB/C mice bearing C26 tumour after iv administration of a single dose of 10 mg/kg on day 14 after tumour inoculation. (a) percent of changes in animal body weight, (b) survival curve, and (c) average tumour volume

Article Snippet: C26 mouse colon carcinoma cells were acquired from Cell Lines Service (Eppelheim, Germany), and they were cultured in Stands for Roswell Park Memorial Institute Medium (RPMI) 1640 medium (Sigma‐Aldrich).

Techniques:

Journal: IET Nanobiotechnology

Article Title: The role of size in PEGylated liposomal doxorubicin biodistribution and anti‐tumour activity

doi: 10.1049/nbt2.12094

Figure Lengend Snippet: Therapeutic efficacy data of different PEGylated nanoliposomal formulations, Caelyx® and free Dox in BALB/C mice bearing C26 tumour. Data represented as mean ± standard deviation ( n = 5)

Article Snippet: C26 mouse colon carcinoma cells were acquired from Cell Lines Service (Eppelheim, Germany), and they were cultured in Stands for Roswell Park Memorial Institute Medium (RPMI) 1640 medium (Sigma‐Aldrich).

Techniques: Standard Deviation

Journal: IET Nanobiotechnology

Article Title: The role of size in PEGylated liposomal doxorubicin biodistribution and anti‐tumour activity

doi: 10.1049/nbt2.12094

Figure Lengend Snippet: Biodistribution of different PEGylated nanoliposomal formulations, Caelyx® and free Dox in organs including (a) Tumour (b) heart, (c) spleen, (d) liver, (e) kidney and (f) lung in BALB/c mice bearing C26 tumour after a single dose of 10 mg/kg administered iv 14 days after the tumour inoculation. Data expressed as mean ± S.E.M. Statistically significant differences are shown as follows: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000.1

Article Snippet: C26 mouse colon carcinoma cells were acquired from Cell Lines Service (Eppelheim, Germany), and they were cultured in Stands for Roswell Park Memorial Institute Medium (RPMI) 1640 medium (Sigma‐Aldrich).

Techniques:

Journal: IET Nanobiotechnology

Article Title: The role of size in PEGylated liposomal doxorubicin biodistribution and anti‐tumour activity

doi: 10.1049/nbt2.12094

Figure Lengend Snippet: Biodistribution of different PEGylated nanoliposomal formulations, Caelyx® and free Dox at different time points in blood in BALB/c mice bearing C26 tumour after a single dose of 10 mg/kg administered iv 14 days after the tumour inoculation. Data expressed as mean ± SEM

Article Snippet: C26 mouse colon carcinoma cells were acquired from Cell Lines Service (Eppelheim, Germany), and they were cultured in Stands for Roswell Park Memorial Institute Medium (RPMI) 1640 medium (Sigma‐Aldrich).

Techniques:

Journal: IET Nanobiotechnology

Article Title: The role of size in PEGylated liposomal doxorubicin biodistribution and anti‐tumour activity

doi: 10.1049/nbt2.12094

Figure Lengend Snippet: The plasma pharmacokinetic parameters using non‐compartmental methods in BALB/c mice bearing C26 tumour following intravenous injection of free Dox, Caelyx® and different sizes of PEGylated nanoliposomal formulations at a single dose of 10 mg/kg

Article Snippet: C26 mouse colon carcinoma cells were acquired from Cell Lines Service (Eppelheim, Germany), and they were cultured in Stands for Roswell Park Memorial Institute Medium (RPMI) 1640 medium (Sigma‐Aldrich).

Techniques: Injection

Journal: Cell reports

Article Title: Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation

doi: 10.1016/j.celrep.2021.109587

Figure Lengend Snippet: (A) Representative confocal images of bone-marrow-derived macrophages (BMDMs, magenta) incubated with MC38-hHER2 cells (green), either with or without tumor targeting anti-hHER2 antibody opsonization. White arrows within insets indicate phagocytosed MC38-hHER2 cells. Scale bar is 50 μm. (B) Quantification of MC38-hHER2 tumor cell phagocytosis by BMDMs. MC38-hHER2 tumor cells were opsonized with a range of anti-hHER2 antibody concentrations (0–5 μg/mL). Each condition is the average of three independent experiments representing >150 total cells. Bars represent means ± SEM. Conditions were compared with two-tailed Student’s t test. **p < 0.01, ***p < 0.001. (C) Fluorescent confocal images of fixed BMDMs engaging with MC38-hHER2 cells (green) opsonized with different concentrations of anti-hHER2 antibody (0,0.5, and 5 μg/mL, magenta). Phosphorylation visualized via anti-phosphotyrosine (red). BMDM outlines are shown in white dotted lines. Areas of high anti-hHER2 enrichment are highlighted with white arrows. Scale bar is 5 μm. (D) Anti-SIRPα blocking antibody 18a interrupting SIRPα binding to CD47 (left panel). MC38-hHER2 cells incubated with a range of anti-hHER2 antibody concentrations (0–5 μg/mL) were added to BMDMs in the presence or absence of 5 μg/mL of 18a antibody. Average tumor cell phagocytosis was quantified (right panel). Each condition is the average of three independent experiments representing >150 total cells. Bars represent means ± SEM. Conditions were compared with two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: MC38 mouse colon carcinoma cell line , Aduro Biotech , N/A.

Techniques: Derivative Assay, Incubation, Two Tailed Test, Phospho-proteomics, Blocking Assay, Binding Assay

Journal: Cell reports

Article Title: Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation

doi: 10.1016/j.celrep.2021.109587

Figure Lengend Snippet:

Article Snippet: MC38 mouse colon carcinoma cell line , Aduro Biotech , N/A.

Techniques: Mutagenesis, Blocking Assay, Virus, Recombinant, Cell Culture, Derivative Assay, Plasmid Preparation, Software